Whole-body 18FDG PET plus pelvic MRI in the pre-treatment assessment of cervical cancers: an alternative to the FIGO clinical staging
© Springer-Verlag Berlin / Heidelberg 2004
Published: 11 March 2004
Growing evidence indicates that whole-body 18F-fluorodeoxyglucose positron emission tomography (wb-18FDG PET) plus pelvic magnetic resonance imaging (pMRI) may significantly improve the pre-treatment staging of primary cervical cancers. Such a combined protocol provides complementary insights into primary tumour delineation, loco-regional involvement and distant spread. As such, pMRI appears particularly reliable for the accurate measurement of tumour size, the detection of parametrial invasion and, even more so, for its exclusion. So far, wb-18FDG PET yields unique information about extra-pelvic nodal and visceral tumour status. Of note, however, is the limitation of both imaging techniques for the detection of microscopic pelvic lymph node metastases, especially in early stage disease. Promising data also highlight the prognostic value of 18FDG uptake as a marker of disease aggressiveness and of tumour resistance to treatment. The recent development of combined PET-CT scans as well as the validation of the sentinel node concept in gynaecological malignancies may grant new perspectives for optimal management of cervical cancers in the pre-treatment setting.
Despite an earlier diagnosis of pre-invasive forms of uterine cancers, the appropriate staging of invasive cervical cancers is still a challenging area . In the pre-treatment setting, the International Federation of Gynaecology and Obstetrics (FIGO) clinical staging system is the most often used classification worldwide . Yet, regardless of FIGO staging, major discrepancies exist when compared to surgical staging . For instance, the underestimation of the pathological extent of disease has been shown to reach nearly one-third of patients with FIGO stage IB and half of those with FIGO stages II–IV [4, 5]. Also, nodal involvement, one of the most important prognostic factors, is not taken into account in the FIGO classification . Taken together, these facts have led a number of clinicians to recommend the use of the TNM classification, which refers to the extent of the primary tumour (T), lymph node metastasis (N) and distant metastasis (M), instead of the conventional FIGO clinical staging . On the other hand, critical changes in treatment planning of newly diagnosed cervical cancers have opened promising perspectives in terms of survival, thereby stressing the need for an accurate staging of disease in the process of patient selection [7, 8, 9].
Current and future technology for staging primary cervical cancers. MRI magnetic resonance imaging, 18 FDG 18F-fluorodeoxyglucose, PET positron emission tomography, CT computed tomography, SNB sentinel node biopsy, 60 Cu-ATSM 60Cu-diacetyl-bis (N4-metylthiosemicarbazone), 18 F-FMISO 18F-fluoromisonidazole, USPIO MRI ultra small paramagnetic iron oxide-enhanced magnetic resonance imaging
The accurate characterisation of the primary tumour is a key step in the pre-treatment staging of cervical cancers. This includes the detection of the initial tumour site and its precise delineation in a three-dimensional space. Also critical is the assessment of prognostic variables such as the depth and width of stromal invasion, local extension into the vagina or the parametrium, as well as the spread of the tumour into the pelvic wall and beyond into the bladder and/or the rectum [1, 2, 13, 15].
In the assessment of primary cervical cancers, the value of MRI is well documented [4, 5, 16, 17]. More recently, increasing amounts of data also have shown that 18FDG PET may detect an intense metabolic signal at the level of the primary site with a high sensitivity (81–100%) [10, 11, 12, 13, 14, 18, 19, 20]. When the performances of both imaging modalities were compared in a prospective series including 22 patients with histologically proven cervical cancers, a similar sensitivity of 91% was found. Microscopic lesions (FIGO IA–T1A), however, were missed by MRI, and by 18FDG PET as well . On the other hand, only pMRI was able to provide precise assessment of the primary tumour size, which correlated well with the pathological findings. Similarly, MRI has yielded valuable information about the extent of the local tumour in the vagina, the parametrium and the pelvic organs, whereas metabolic imaging could not assess such key parameters owing to its limited spatial resolution. Of interest, morphological imaging was even more accurate to rule out a parametrial involvement, thereby giving a high negative predictive value. In a few cases, however, the inflammation surrounding the primary tumour site as well as the acute oedema following a recent biopsy gave a high MRI signal, which was falsely interpreted as a tumour. In summary, even though MRI and 18FDG PET may detect cervical cancers with a high sensitivity, morphological imaging is better indicated for primary tumour characterisation and loco-regional staging. Hence, MRI appears to be the modality of choice for the T staging of cervical cancers.
In cervical cancers, nodal status is a major independent prognostic variable [1, 4, 15, 17]. In women presenting with nodal involvement (N1), the 5-year overall survival is dramatically reduced by nearly 30–40% in comparison to that of patients classified as N0 [22, 23, 24]. In particular, para-aortic nodal status has been shown to be the most powerful parameter for patient outcomes [25, 26]. On the other hand, the recent introduction of combined therapies including radiation therapy plus sensitising chemotherapy gives rise to rational hopes in terms of prolonged survival [27, 28, 29]. So far, among the available diagnostic tools that are recommended by the FIGO staging system for the assessment of nodal status, neither lymphangiography, computed tomography (CT) nor MRI are sensitive enough for the accurate detection of nodal metastases [2, 4, 5, 30]. In this particular clinical context, 18FDG PET may be the best alternative for highly sensitive whole-body imaging, which allows the localisation of metastases at each level of the natural history of nodal spreading. Indeed, many clinical reports have shown the added value of metabolic imaging for detecting lymph node metastases at the pelvic and extra-pelvic levels [10, 14, 18, 19, 20, 21]. Quiet frequently, 18FDG PET alone was able to detect nodal involvement missed by the CT and/or MRI [18, 19, 20, 21, 31]. More importantly, 18FDG uptake at the para-aortic level was found the most significant factor for disease-free survival . Like other reports, our experience confirms the superiority of metabolic imaging to MRI for the detection of para-aortic, mediastinal and supra-clavicular lymph node metastases [18, 19, 20, 31, 32]. Unlike many series, however, our data highlight the technical limitations of 18FDG PET for the evaluation of pelvic metastases, lymph node sites that most often harbour microscopic involvement and may even escape the intra-operative palpation [21, 33, 34]. Hence, 18FDG PET appears to be the modality of choice for achieving an accurate N staging in patients with cervical cancers. Care should be taken, however, in the assessment of pelvic metastases that are below the spatial resolution of commercially available PET scanners.
The staging of distant metastases is critical for treatment decision-making. Although lung, liver and bone metastases are the most frequent sites of secondary localisations arising from cervical cancers, results from large surgical series and autopsy studies showed that a large spectrum of organs may be affected with a variable frequency . Also, 18FDG PET appears particularly appropriate for the detection of distant tumour sites through the entire body [13, 14, 32, 36]. Interestingly, cervical cancers exhibit high rates of GLUT-1 (type-1 glucose transporter) and glycolytic enzymes (type-2 hexokinase, phosphofructokinase and pyruvate kinase), which makes the imaging by means of the glucose analogue theoretically and practically valid for such gynaecological malignancies [37, 38, 39, 40, 41]. In addition, metabolic imaging offers the possibility of non-invasive whole-body scanning in a single session, whereas the FIGO staging system recommends a number of radiological and endoscopic studies, iterative explorations that are not only invasive and expensive, but often clinically fruitless [2, 3, 4, 5]. Our data are in line with the literature results, which highlight the added value of 18FDG PET in detecting visceral metastases missed by morphological imaging (CT/MRI). Of note is the suboptimal specificity of 18FDG for tumour cell uptake  so that, in a particular clinical context, inflammatory or infectious diseases may avidly take up the glucose tracer, thereby leading to false-positive results [42, 43]. In the M staging of cervical cancers, growing evidence indicates the usefulness of 18FDG PET for more appropriate treatment planning. In agreement with some authors, we advocate the introduction of metabolic imaging in the initial work-up of women presenting with cervical cancers [13, 14, 21, 32]. So far, more data need to be gleaned in order to refine the correct place of 18FDG PET on a stage-by-stage basis.
In most papers published, the use of 18FDG PET in the work-up of cervical cancers significantly altered the treatment options . The rates of treatment impact vary from 14 to 60% depending on the stage of disease, the extent of the lesions detected and ultimately the modalities of treatments initially selected [20, 21, 31, 32, 44]. On average, one-third of patients presenting with various stages of cervical cancers may draw a direct benefit from metabolic imaging alone, a treatment adjustment resulting from a more accurate staging. Para-aortic metastases are the most documented 18FDG-avid sites where metabolic imaging best influences the treatment choices [10, 14, 18, 20, 21, 31, 32]. Indeed, in many clinical data, 18FDG PET modified the fields, volumes and doses of irradiation by detecting unsuspected para-aortic nodal involvement. Metabolic imaging was also found to be the best technique for the detection of inpalpable supra-clavicular metastases, thereby reorienting the conventional therapies to a palliative therapy or irradiation plus concurrent chemotherapy . Similarly, the 18FDG findings may guide the treatment by the detection of visceral metastases (lung, liver, skeleton), which are often overlooked by the routine protocols [14, 21, 36]. Results from a combined protocol including wb-18FDG PET plus pMRI are in line with the data in the literature. In approximately 20% of patients (4/22), the aforementioned imaging work-up significantly modified the treatment options from conservative surgery to chemo-radiation, including the para-aortic fields (three patients upstaged from FIGO-N0M0 to PET-N1M0 and PET-N1M1) or to radiation therapy with a bony pelvic boost (one patient upstaged from FIGO-M0 to PET-M1) . Importantly, in all patients, the PET findings were confirmed either by histology or by clinical and CT follow-up; among them, 80% of women had a surgical confrontation with nodal disease. In addition, the agreement scores of 18FDG PET with the final diagnosis were significantly higher than that of FIGO staging, including pMRI (P<0.01). On the other hand, although pMRI upstaged the local extent of disease in five patients (three patients from T2a to T2b, one patient from T3a to T3b and one patient from T3a to T4), results from surgical exploration and the final pathological analysis showed that only two of them had parametrial involvement; the other cases were considered to be false-positive results resulting from peri-tumoral inflammatory changes or artefact-related MRI signals. Ultimately, in the four patients with a positive PET study, metabolic imaging directly influenced the treatment decision-making. Conversely, based on the positive MRI results, the therapeutic decision was always defined a posteriori during surgery or following the pathological conclusions. So far, in all patients but one, a negative MRI was accurately predictive of no disease at the level of the parametrium (T2a vs. T2b), the vagina (T3a vs. T3b) and the bladder/rectum (T4). In conclusion, whether the disease is locally confined or already advanced, the appropriate combination of 18FDG PET and MRI in pre-treatment staging of cervical cancers may help make the choice of the best strategy in terms of maximal versus minimal treatments.
Even though a pre-treatment work-up including wb-18FDG PET plus pMRI may significantly improve the staging of primary cervical cancers, some questions are still pending. First, further studies to assess the cost effectiveness of such a combined protocol in comparison to the routine protocols are needed. This is a key point to consider for a large scale implementation, knowing that nearly 80% of cervical cancers occur in developing countries . Second, large controlled trials are still needed in terms of adequate population sampling, defining a robust gold standard and sufficient follow-up. Despite the increasing number of clinical reports, the best methodological and analytical criteria are not always met . In particular, the pathological confirmation of the imaging findings (18FDG PET, MRI) is a prerequisite when assessing the value of both modalities for nodal staging. The accurate determination of the predictive values of 18FDG PET and MRI (presence of disease when the test is positive and absence of disease when the test is negative) is implicit. Otherwise, owing to the suboptimal performances of the conventional clinical and radiological confrontation, no definitive conclusion can be drawn objectively. Third, metabolic imaging is not just a diagnostic modality, but it may also bring prognostic information to bear upon tumour behaviour and patient survival [32, 47]. Also, the inclusion of homogenous groups of patients based on the TNM/FIGO staging systems is critical to a precise stage-by-stage determination of the best indications of wb-18FDG PET plus pMRI along with the merging of new technologies . Last but not least, a multidisciplinary approach to the disease in terms of clinical demand and technology supply should be preferred to optimise the protocol proposed for the pre-treatment staging of cervical cancers. At this level, the role of international medical societies, which are primarily involved in this area of clinical investigation (i.e. gynaecology, radiology, pathology and nuclear medicine), appears crucial.
The field of molecular imaging is continuously evolving. Combined imaging technologies, more specific tracers, as well as improved histopathological techniques are available nowadays in the clinical setting to optimise the staging of cervical cancers. At the same time, the recent introduction of PET-CT devices have allowed the assessment of the extent of the disease over the entire body [48, 49]. In previous studies, the software fusion of PET and CT images was already found useful for the accurate determination of the target primary tumour volume in patients who were candidates for radiation therapy [50, 51]. The hardware fusion of PET and CT images may significantly improve the diagnostic accuracy of both imaging techniques, thereby giving an anato-metabolic picture of the disease . In gynaecological cancers, besides a more precise detection of disease, 18FDG PET-CT may impact the treatment options in nearly 30% of the patients compared to 18FDG PET alone [53, 54]. Similarly, the development of moving patient platforms with integrated surface-coil technology has recently enabled the performance of whole-body MRI within a single session. In a comparative study performed in 98 patients with various cancers including genitourinary tumours, 18FDG PET-CT was found more accurate for the T and the N staging, whereas whole-body MRI may be the best modality for the M staging, especially for the detection of liver and bone metastases . Also promising is the use of hypoxia tracers in PET imaging [56, 57]. Because hypoxia is an important prognostic factor in cervical cancers, the use of 18F-FMISO (18F-fluoromisonidazole) or 60Cu-ATSM [60Cu-diacetyl-bis (N4-metylthiosemicarbazone)] as hypoxia tracers may give the clinicians determining information on tumour components . These tracers also may be particularly useful for the initial assessment of tumour responsiveness to treatment, knowing that hypoxic cells are rather resistant to radiotherapy and chemotherapy . On the other hand, the validation of the sentinel node concept in early stage cervical cancers along with the molecular analysis of the sentinel lymph nodes (SLN) may grant new perspectives for the selective evaluation of regional nodal status [60, 61, 62]. Accordingly, most women with early stage disease and negative SLN will be spared the morbidity resulting from unnecessary complete lymph node dissections. Conversely, the detection of SLN with microscopic involvement, especially in unpredictable sites, may radically change the treatment strategy. Similarly, high-resolution MRI using lymphotropic paramagnetic iron oxide nanoparticles instead of conventional gadolinium-enhanced MRI may significantly improve the detection of lymph node metastases without the need of invasive investigations [63, 64, 65]. In other words, in women suffering from cervical cancers, the development of new technologies as well as the improvement of diagnostic tools already available routinely may help overcome the limitations of the current staging systems. Further, clinical studies are still needed to confirm these encouraging preliminary data.
The staging of primary cervical cancers may be significantly improved by combining whole-body 18FDG PET plus pelvic MRI. Accordingly, MRI is the modality of choice for the T staging of disease, whereas 18FDG PET is better indicated for the assessment of nodal involvement (N staging) and distant metastases (M staging). Further controlled trials are still needed to assess the cost-effectiveness of such a protocol, especially to make its clinical added value more precise on a stage-by-stage basis. Similarly, the introduction of new technologies stresses the need for evaluating the best protocol in the pre-treatment setting. These are prerequisite before incorporating a high-end imaging work-up into the international gynaecological classifications.
The author thanks Dr. Pierre Rigo for helpful discussions. Many thanks also go to Drs. Frédéric Kridelka, Alain Thille and Viviana Fridman for useful collaboration.
- Chi DS, Lanciano RM, Kudelka AP (2001) Cancer management: a multidisciplinary approach to cervical cancer, 5th edn. PRR, Inc, New YorkGoogle Scholar
- Benedet JL, Bender H, Jones H 3rd, Ngan HY, Pecorelli S (2000) FIGO staging classifications and clinical practice guidelines in the management of gynecologic cancers. FIGO Committee on Gynecologic Oncology. Int J Gynaecol Obstet 70:209–262Google Scholar
- van Nagell J, Roddick JW, Lowin DM (1971) The staging of cervical cancer: inevitable discrepancies between clinical staging and pathologic findings. Am J Obstet Gynecol 110:973–978Google Scholar
- Hricak H, Yu K (1996) Radiology in invasive cervical cancer. A J R 167:1101–1108Google Scholar
- Yu KK, Forstner R, Hricak H (1997) Cervical carcinoma: role of imaging. Abdom Imaging 22:208–215Google Scholar
- Hermanek P (2002) Why TNM system for staging of gynaecological tumours ? CME J Gynecol Oncol 42:31–40Google Scholar
- Grigsby PW, Herzog TJ (2001) Current management of patients with invasive cervical carcinoma. Clin Obstet Gynecol 44:531–537Google Scholar
- Landoni F, Maneo A, Colomb A, Placa F, Milani R, Perego P, Favini G, Ferri L, Mangioni C (1997) Randomized study of radical surgery versus radiotherapy for stage Ib-IIa cervical cancer. Lancet 350:535–540Google Scholar
- Rose PG, Bundy BN, Watkins EB, Thigpen JT, Deppe G, Maiman MA, Clarke-Pearson DL, Insalaco S (1999) Concurrent cisplatin-based chemoradiation improves progression free and overall survival in advance cervical cancer: results of a randomized gynecology oncology group study. N Eng J Med 340:1144–1153Google Scholar
- Rose PG, Adler LP, Rodriguez M, Faulhaber PF, Abdul-Karim FW, Miraldi F (1999) Positron emission tomography for evaluating para-aortic nodal metastasis in locally advanced cervical cancer before surgical staging: a surgicopathologic study. J Clin Oncol 17:41–45Google Scholar
- Grigsby PW, Dehdashti F, Siegel BA (1999) FDG-PET Evaluation of Carcinoma of the Cervix. Clin Positron Imaging 2:105–109Google Scholar
- Sugawara Y, Eisbruch A, Kosuda S, Recker BE, Kison PV, Wahl RL (1999) Evaluation of FDG PET in patients with cervical cancer. J Nucl Med 40:1125–1131Google Scholar
- Follen M, Levenback CF, Grigsby PW, Delpassand ES, Fornage BD, Fishman EK (2003) Imaging of cervical cancer. Cancer 98 [Suppl 9]:2028–2038Google Scholar
- Belhocine T, Kridelka F, Thille A, De Barsy C, Foidart-Willems J, Hustinx R, Rigo P (2003) Staging of primary cervical cancers: the role of nuclear medicine. Crit Rev Oncol Hematol 46:275–284Google Scholar
- Drain PK, Holmes KK, Hughes JP, Koutsky LA (2002) Determinants of cervical cancer rates in developing countries. Int J Cancer 100:199–205Google Scholar
- Subak LL, Hricak H, Powell CB, Azizi L, Stern JL (1995) Cervical carcinoma: computed tomography and magnetic resonance imaging for preoperative staging. Obstet Gynecol 86:43–50Google Scholar
- Hricak H, Powell CB, Yu KK, Washington E, Subak LL, Stern JL, Cisternas MG, Arenson RL (1996) Invasive cervical carcinoma: role of MR imaging in pretreatment work-up—cost minimization and diagnostic efficacy analysis. Radiology 198:403–409Google Scholar
- Reinhardt MJ, Ehritt-Braun C, Vogelgesang D (2001) Metastatic lymph nodes in patients with cervical cancer: detection with MR imaging and FDG PET. Radiology 218:776–782Google Scholar
- Kühnel G, Horn LC, Fischer U, Fischer U, Hesse S, Seese A, Georgi P, Kluge R (2001)18F-FDG positron-emission tomography in cervical carcinoma: preliminary findings. Zentralbl Gynakol 123:229–235Google Scholar
- Narayan K, Hicks RJ, Jobling T, Bernshaw D, McKenzie AF (2001) A comparison of MRI and PET scanning in surgically staged loco-regionally advanced cervical cancer: potential impact on treatment. Int J Gynecol Cancer 11:263–271Google Scholar
- Belhocine T, Thille A, Fridman V, Albert A, Seidel L, Nickers P, Kridelka F, Rigo P (2002) Contribution of whole-body18FDG PET imaging in the management of cervical cancer. Gynecol Oncol 87:90–97Google Scholar
- Buchsbaum HJ (1979) Extrapelvic lymph node metastasis in cervical carcinoma. Am J Obstet Gynecol 133:814–824Google Scholar
- Tanaka Y, Sawada S, Murata T (1984) Relationship between lymph node metastases and prognosis in patients irradiated postoperatively for carcinoma of the uterine cervix. Acta Radiol Oncol 23:455–459Google Scholar
- Anderson MC, Coulter CAE, Mason WP, et al (1997) Malignant disease of the cervix. In: Shaw RW, Soutter WP, Stanton SI (eds) Gynecology, 2nd edn. Churchill Livingstone, New York, pp 541–568Google Scholar
- Stehman FB, Bundy BN, DiSaia PJ, Keys HM, Larson JE, Fowler WC (1991) Carcinoma of the cervix treated with radiation therapy. I. A multi-variate analysis of prognostic variables in the Gynecologic Oncology Group. Cancer 67:2776–2785Google Scholar
- Heller PB, Malfetano JH, Bundy BN, Barnhill DR, Okagaki T (1990) Clinical-pathological study of stage IIB, III and IVA carcinoma of the cervix: extended diagnostic evaluation for paraaortic node metastases—a Gynecologic Oncology Group study. Gynecol Oncol 38:425–430Google Scholar
- Morris M, Eifel PJ, Lu J, Grigsby PW, Levenback C, Stevens RE, Rotman M, Gershenson DM, Mulch DG (1999) Pelvic radiation with concurrent chemotherapy compared with pelvic and para-aortic radiation for high risk cervical cancer. N Engl J Med 340:1137–1143Google Scholar
- Varia MA, Bundy BN, Deppe G, Mannel R, Averette HE, Rose PG, Connelly P (1998) Cervical carcinoma metastatic to para-aortic nodes: extended field radiation therapy with concomitant 5-fluorouracil and cisplatin chemotherapy: a Gynecologic Oncology Group study. Int J Radiat Oncol Biol Phys 42:1015–1023Google Scholar
- Stryker JA, Mortel R (2000) Survival following extended field irradiation in carcinoma of cervix metastatic to para-aortic lymph nodes. Gynecol Oncol 79:399–405Google Scholar
- Scheidler J, Hricak H, Yu KK, Subak L, Segal MR (1997) Radiological evaluation of lymph node metastases in patients with cervical cancer—a meta-analysis. JAMA 278:1096–1101Google Scholar
- Yeh LS, Hung YC, Shen YY, Kao CH, Lin CC, Lee CC (2002) Detecting para-aortic lymph nodal metastasis by positron emission tomography of18F-fluorodeoxyglucose in advanced cervical cancer with negative magnetic resonance imaging findings. Oncol Rep 9:1289–1292Google Scholar
- Grigsby PW, Siegel BA, Dehdashti F (2001) Lymph node staging by positron emission tomography in patients with carcinoma of the cervix. J Clin Oncol 19:3745–3749Google Scholar
- Williams AD, Cousins C, Soutter WP, Mubashar M, Peters AM, Dina R, Fuchsel F, McIndoe GA, deSouza NM (2001) Detection of pelvic lymph node metastases in gynecologic malignancy: a comparison of CT, MR Imaging and Positron Emission Tomography. AJR 177:343–348Google Scholar
- Benedetti-Panici P, Maneschi F, Scambia G, Greggi S, Cutillo G, D’Andrea G, Rabitti C, Coronetta F, Capelli A, Mancuso S (1996) Lymphatic spread of cervical cancer: an anatomical and pathological study based on 225 radical hysterectomies with systematic pelvic and aortic lymphadenectomy. Gynecol Oncol 62:19–24Google Scholar
- Fulcher AS, O’Sullivan SG, Segreti EM, Kavanagh BD (1999) Recurrent cervical carcinoma: typical and atypical manifestations. Radiographics 19:103–116Google Scholar
- Kerr IG, Manji MF, Powe J, Bakheet S, Al Suhaibani H, Subhi J (2001) Positron emission tomography for the evaluation of metastases in patients with carcinoma of the cervix: a retrospective review. Gynecol Oncol 81:477–480Google Scholar
- Mendez LE, Manci N, Cantuaria G, Gomez-Marin O, Penalver M, Braunschweiger P, Nadji M (2002) Expression of glucose transporter-1 in cervical cancer and its precursors. Gynecol Oncol 86:138–143Google Scholar
- Yen TC, See LC, Lai CH, Yah-Huei CW, Ng KK, Ma SY, Lin WJ, Chen JT, Chen WJ, Lai CR, Hsueh S (2004)18F-FDG uptake in squamous cell carcinoma of the cervix is correlated with glucose transporter 1 expression. J Nucl Med 45:22–29Google Scholar
- Marshall MJ, Goldberg DM, Neal FE, Millar DR (1978) Enzymes of glucose metabolism in carcinoma of the cervix and endometrium of the human uterus. Br J Cancer 37:990–1001Google Scholar
- Marshall MJ, Neal FE, Goldberg DM (1979) Isoenzymes of hexokinase, 6-phosphogluconate deshydrogenase, phosphoglucomutase and lactate deshydrogenase in uterine cancer. Br J Cancer 40:380–390Google Scholar
- Kikuchi Y, Sato S, Sugimura T (1972) Hexokinase isozyme patterns of human uterine tumors. Cancer 30:444–446Google Scholar
- Shreve PD, Anzai Y, Wahl RL (1999) Pitfalls in oncologic diagnosis with FDG PET imaging: physiologic and benign variants. Radiographics 19:61–77Google Scholar
- Kubota R, Yamada S, Kubota K, Ishiwata K, Tamahashi N, Ido T (1992) Intratumoral distribution of fluorine-18-fluorodeoxyglucose in vivo: high accumulation in macrophages and granulation tissues studied by microautoradiography. J Nucl Med 33:1972–1980Google Scholar
- Talbot JN, Grahek D, Kerrou K, Younsi N, de Beco V, Colombet-Lamau C, Petegnief Y, Cailleux N, Montravers F (2001) La TEP au 18F-fluoro-2-déoxyglucose dans l’imagerie des cancers gynécologiques. Gynécol Obstét Fertil 29:775–798Google Scholar
- Tran BN, Grigsby PW, Dehdashti F, Herzog TJ, Siegel BA (2003) Occult supraclavicular lymph node metastasis identified by FDG-PET in patients with carcinoma of the uterine cervix. Gynecol Oncol 90:572–576Google Scholar
- Parkin DM, Bray F, Ferlay J, Pisani P (2001) Estimating the world cancer burden: Globocan 2000. Int J Cancer 94:153–156Google Scholar
- Singh AK, Grigsby PW, Dehdashti F, Herzog TJ, Siegel BA (2003) FDG-PET lymph node staging and survival of patients with FIGO stage IIIb cervical carcinoma. Int J Radiat Oncol Biol Phys 56:489–493Google Scholar
- Cheng-chien T, Chien-Sheng T, Koon-kwan Ng, Chyong-huey L, Swei H, Pan-Fu K, Ting-Chang C, Ji-Hong H, Tzu-Chen Y (2003) The impact of image fusion in resolving discrepant findings between FDG-PET and MRI/CT in patients with gynaecological cancers. Eur J Nucl Med Mol Imag 30:1674–1683Google Scholar
- Mutic S, Grigsby MS, Low DA, Dempsey JF, Harms WB, Laforest R, Bosch WR, Miller T (2001) PET-guided three-dimensional treatment planning of intracavitary gynecologic implants. Int J Radiat Oncol Biol Phys 52:1104–1110Google Scholar
- Miller TR, Grigsby PW (2002). Measurement of tumor volume by PET to evaluate prognosis in patients with cervical cancer. Int J Radiat Oncol Biol Phys 53:353–359Google Scholar
- Malyapa RS, Mutic S, Low DA, Zoberi I, Bosch WR, Laforest R, Miller TR, Dempsey JF, Grigsby MW (2002) Physiologic FDG-PET three-dimensional brachytherapy treatment planning for cervical cancer. Int J Radiat Oncol Biol Phys 54:1140–1146Google Scholar
- Cohade C, Wahl RL (2003) Applications of positron emission tomography/computed tomography image fusion in clinical positron emission tomography—clinical use, interpretation methods, diagnostic improvements. Semin Nucl Med 23:228−237Google Scholar
- Wahl RL (2004) Why nearly all PET of abdominal and pelvic cancers will be performed as PET/CT. J Nucl Med 45:82S–95SGoogle Scholar
- Czernin J (2004) Summary of selected PET/CT abstracts from the 2003 Society of Nuclear Medicine Annual Meeting. J Nucl Med 45:102S–103SGoogle Scholar
- Antoch G, Vogt FM, Freudenberg LS, Nazaradeh F, Gohde SC, Barkhausen J, Dahmen G, Bockisch A, Debatin JF, Ruehm SC (2003) Whole-body dual-modality PET/CT and whole-ody MRI for tumour staging in oncology. JAMA 290:3199–3206Google Scholar
- Koh WJ, Rasey JS, Evans ML (1992) Imaging of hypoxia in human tumors with [18F]fluoromisonidazole. Int J Radiat Oncol Biol Phys 22:199–212Google Scholar
- Fujibayashi Y, Taniuchi H, Yonekura Y, Ohtani H, Konishi J, Yokoyama A (1997) Copper-62-ATSM: a new hypoxia imaging agent with high membrane permeability and low redox potential. J Nucl Med 38:1155–1160Google Scholar
- Dehdashti F, Mintun MA, Lewis JS, Bradley J, Gocindan R, Laforest R, Welch MJ, Siegel BA (2003) In vivo assessment of tumor hypoxia in lung cancer with60Cu-ATSM. Eur J Nucl Med Mol Imag 30:844−850Google Scholar
- Dehdashti F, Grigsby PW, Mintun MA, Lewis JS, Siegel BA, Welsh MJ (2003) Assessing tumor hypoxia in cervical cancers by positron emission tomography with (60)Cu-ATSM: relationship to therapeutic response-a preliminary report. Int J Radiat Oncol Biol Phys. 55:1233–1238Google Scholar
- Levenback C, Coleman RL, Burke TW, Lin WM, Erdman W, Deavers M, Delpassand ES (2002) Lymphatic mapping and sentinel node identification in patients with cervix cancer undergoing radical hysterectomy and pelvic lymphadenectomy. J Clin Oncol 20:688–693Google Scholar
- Rhim CC, Park JS, Bae SN, Namkoong SE (2002) Sentinel node biopsy as an indicator for pelvic nodes in early stage cervical cancer. J Korean Med Sci 17:507–511Google Scholar
- Van Trappen PO, Gyselman VG, Lowe DG, Ryan A, Oram DH, Bosze P, Weekes AR, Shepherd JH, Dorudi S, Bustin SA, Jacobs IJ (2001) Molecular quantification and mapping of lymph-node micrometastases in cervical cancer. Lancet 357:15–20Google Scholar
- Weissleder R, Elizondo G, Wittenberg J, Lee AS, Josephson L, Brady TJ (1990) Ultra-small superparamagnetic iron oxide: an intravenous contrast agent for assessing lymph nodes with MR imaging. Radiology 175:494–498Google Scholar
- Harisinghani MG, Saini S, Weissleder R, et al (1999) MR lymphangiography using ultrasmall superparamagnetic iron oxide in patients with primary abdominal and pelvic malignancies: radiographic-pathologic correlation. AJR Am. J Roentgenol 172:1347–1351Google Scholar
- Harisinghani MG, Barentsz J, Hahn PF, Deserno WM, Tabatabaei S, van de Kaa CH, de la Rosette J, Weissleder R (2003) Noninvasive detection of clinically occult lymph-node metastases in prostate cancer. N Engl J Med 348:2491–2499Google Scholar